Cloning and expression of the tumor-associated antigen L6.

Tumor-associated antigen L6 and the invasion of human lung cancer cells.

Metastasis is a coordinated process that depends on the interaction of cancer cells with the tumor microenvironment. Members of the transmembrane-4 superfamily (TM4SF) of surface proteins have been implicated in the regulation of cancer cell metastasis, and the expression of several TM4SF members on tumor cells is inversely correlated with patient prognosis. The tumor-associated antigen L6 (TAL...

Cloning and Expression of Leptospira LipL32 Antigen as a Candidate for Rapid Diagnosis

Background and Objective: Leptospirosis as an important emerging infectious zoonotic disease caused by spirochetes of the genus Leptospira. Given the low sensitivity and long duration of its culture, the diagnosis of leptospirosis is mainly based on serological methods. The microscopic agglutination test (MAT) is considered as the reference method. Because of the complexity of the MAT, there is...

cloning and expression of gumboro vp2 antigen in aspergillus niger

background: infectious bursal disease virus (ibdv) causes a highly immunosuppressive disease in chickens and is a pathogen of major economic importance to the poultry industry worldwide. the vp2 protein is the major host-protective immunogen of ibdv and has been considered as a potential subunit vaccine against the disease. vp2 coding sequence was cloned in an inducible fungal vector and the pr...

In silico cloning and bioinformatics study of Brucella melitensis Omp31 antigen in different mammalian expression vectors

Brucella melitensis, as a pathogenic gram-negative intracellular bacterium, causes brucellosis in animals and humans. According to literature, the B. melitensis outer membrane protein 31 (Omp31) is considered as an important vaccine candidate against brucellosis. The aim of the current study was to compare three different expression constructs containing B. melitensis Omp31 antigen using bioinf...

cloning and expression of hepatitis b surface antigen